Wie man ein genetisch verändertes Virus herstellt:
Geschrieben von Henzo am 11. April 2020 17:31:19:
Als Antwort auf: Re: Schwachsinn geschrieben von Hanomedes am 11. April 2020 16:57:21:
MATERIALS AND METHODS
Cells.Vero cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 IU/ml penicillin, and 100 μg/ml streptomycin (1% P/S; Mediatech Inc., Manassas, VA, USA). BHK21 cells were maintained in DMEM containing 10% tryptose phosphate broth (TPB), 10% fetal bovine serum (FBS), 100 IU/ml penicillin, and 100 μg/ml streptomycin. MDBK cells were grown in DMEM containing 5% FBS and 1% P/S. Cells were prepared 1 day prior to infection, achieving approximately 90% confluence by the following day.
Viruses.The plasmid containing the cDNA clone of PIV5 with MERS-S inserted between SH and HN was constructed using previously described methods (22, 26, 29). Primer sequences are available upon request. Infectious virus was rescued in BHK cells as previously described (26). Recombinant PIV5 or PIV5-MERS-S was propagated in MDBK cells as previously described (26, 29).
Das geht auch mit menschlichen Zellen.
ciao, HENZO.
- Re: Wie man ein genetisch verändertes Virus herstellt: Hanomedes 11.04.2020 17:57 (3)
- Die Hälfte, steht in dem Bericht. (ohne Text) Henzo 11.04.2020 18:43 (2)
- Re: Die Hälfte, steht in dem Bericht. Hanomedes 11.04.2020 19:25 (1)
- Du hast es also nicht gelesen. (ohne Text) Henzo 12.04.2020 15:15 (0)